Isolation of Periplasmic Alkaline Phosphatase from Rhizobium Bacteria
نویسندگان
چکیده
منابع مشابه
Isolation and molecular identification of bacteria producing alkaline phosphatase enzyme from environmental sources
Alkaline phosphatase (ortho phosphate, monoester hydrolase phosphoinositide E. C. 3.1.3.1) is a non-specific metalloproteinase enzyme that is located inside periplasmic space of bacteria. This enzyme is used to measure freshwater sediment in genetic engineering for cleaning water. Isolation and molecular identification of the bacteria producing alkaline phosphatase and comparison of its product...
متن کاملisolation and molecular identification of bacteria producing alkaline phosphatase enzyme from environmental sources
alkaline phosphatase (ortho phosphate, monoester hydrolase phosphoinositide e. c. 3.1.3.1) is a non-specific metalloproteinase enzyme that is located inside periplasmic space of bacteria. this enzyme is used to measure freshwater sediment in genetic engineering for cleaning water. isolation and molecular identification of the bacteria producing alkaline phosphatase and comparison of its product...
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Large numbers of magnetotactic bacteria were discovered in mud and water samples collected from a number of highly alkaline aquatic environments with pH values of ≈ 9.5. These bacteria were helical in morphology and biomineralized chains of bullet-shaped crystals of magnetite and were present in all the highly alkaline sites sampled. Three strains from different sites were isolated and cultured...
متن کاملDifferential Synthesis of Alkaline Phosphatase in Rhizobium Species Isolated from the Tropics
A total o f 28 strains o f several species o f Rhizobium were screened for the presence and regulation of the enzyme alkaline phosphatase. In contrast to all other species o f Rhizobium, the activity of this enzyme was absent in R. trifolii. D ifferent levels o f alkaline phosphatase were observed in various species o f Rhizobium. Both, species as well as strain specific differences were found ...
متن کاملLocalization of alkaline phosphatase in three gram-negative rumen bacteria.
Of the three species (Bacteroides ruminicola, B. succinogenes, and Megasphaera elsdenii) of anaerobic gram-negative rumen bacteria studied, only B. ruminicola produced significant amounts of alkaline phosphatase. This enzyme, which is constitutive, showed a greater affinity for p-nitrophenylphosphate than for sodium-beta-glycerophosphate and was shown to be located exclusively in the periplasmi...
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ژورنال
عنوان ژورنال: Research Journal of Microbiology
سال: 2008
ISSN: 1816-4935
DOI: 10.3923/jm.2008.157.162